The maximum OOBAR capacity for Mo(V) sorption was 1.3 mmol/g. The molar ratio of Mo(SCN)6-, OOBAR+ was 1:1 in acidic medium. The equilibrium isotherms, kinetics, diffusion and thermodynamic models for Mo(V) sorption onto OOBAR were studied. The parameters of thermodynamic such as enthalpy (?H), entropy (?S) and Gibbs free energy (?G) were -46.2 kJ/mol, -0.082 kJ/Kmol and -21.8 kJ/mol was shown that the sorption process was spontaneous, exothermic nature with decrease disorder and randomness at the solid-liquid interface of Mo with OOBAR. Dynamic experiments using glass column was indicated a good affinity chromatographic separation for its applications in many pharmaceutical and biological samples including liver mice tissue and pharmaceutical vitamin drugs. The lower relative standard deviation (RSD%) value for pharmaceutical applications samples (n=5) was found from 1.1%.