Assignment No

Assignment No.2 of 3Assignment TitleThe liver and metabolism.Date of Internal Verification of assignmentName of Internal Verifier Description of Assessment Tasks 2.1 Part A of UBAR 2.2 Part B of UBAR 2.3 Part C of UBAR Learning OutcomesAssessment Criteria2. Understand the liver and its central role in metabolism.2.1. Illustrate the gross structure and histology of the liver. 2.2. Analyse how the liver contributes to homeostasis, by giving one example each for its involvement in processing of fats, carbohydrates, amino acids and xenobiotics. 2.3. Define excretion and outline the urea cycle. Grading InformationIf you have achieved all Level 3 criteria you will receive a grade (Pass, Merit or Distinction) against the Grade Descriptors below. Just meeting the demands of the learning outcomes you will only be graded pass.Grade Descriptor To achieve a Merit The learner hasTo achieve a Distinction The learner hasGD1aDemonstrated a very good grasp of the relevant knowledge baseDemonstrated an excellent grasp of the relevant knowledge baseGD 2a,bMade use of relevant facts, theories or concepts with either breadth or depthMade use of relevant facts, theories or concepts with both breadth or depthGD 4a,eIdentified new information from sources which are generally appropriate. Generally combined or synthesised information with outcomes that are accurate and appropriate.Identified new information from sources which are consistently appropriate. Consistently combined or synthesised information with outcomes that are accurate, appropriate, succinct, innovative and creative.GD 7c Demonstrated a very good response to the demands of the assignment briefDemonstrated an excellent response to the demands of the assignment briefWhat this means for this assignment To gain merits for this assignment I would expect your work to be factually correct (GD1 2) and written in full sentences with appropriate use of relevant scientific keywords and referencing (GD1 4). For distinctions I would expect your written work to contain evidence of additional reading and research, outside of content provided in class and from appropriate sources (GD 2 4). I would expect the assignment to be handed in on time and presented in a neat and orderly way with all questions completed to the best of your ability (GD7). I would expect that any drawings are neat and labelled appropriately (GD 4, 7). Any pictures drawn or inserted from an appropriate external source should be appropriately referenced with a figure legend (GD4). Declaration of authenticity I confirm that the assignment I have submitted is all my own work Signature of student Date submitted Access to HE Unit BIO 090 Digestion and Excretion (WJF679 G) Learning Outcome LO2 (Liver) To achieve level 3, address the questions correctly. Part A (Assessment Criterion 2.1) The liver. Q1 Dissect a mammalian liver. Prepare labelled drawings to show the gross anatomy of the liver from both aspects. The liver is situated in the abdomen just below the diaphragm (Houston et al., 2016). It consists of 4 distinct lobes the left, right, caudate, and quadrate lobes shown in figure 1, and is the only organ that regenerates itself enabling a partial transplant to be able to have a functioning liver (Houston et al., 2016). Liver cells have large numbers of mitochondria and a large Golgi Apparatus to enable ATP for their energy needs (Houston et al., 2016). Microvilli is also needed to increase the surface area to absorb substances from the blood (Houston et al., 2016). The liver has a vital function in red blood cells formation, it synthesizes the haematinic principle from vitamin B12 which is stored in the liver (Houston et al., 2016). The liver has many functions processing, manufacturing and storage (Houston et al., 2016). Processing The liver makes glucose out of carbohydrates when the body is low on energy (Houston et al., 2016). The liver metabolizes fat this is essential when glucose is running out (Houston et al., 2016). Excess carbohydrates and proteins are converted into fatty acids then release into the blood stream for energy (Houston et al., 2016). The liver filters what we eat, what gets absorbed into our blood and excretes it as solid (feces) and liquid waste (urine) (Houston et al., 2016). The liver converts fat-soluble toxins into a water-soluble substance (Houston et al., 2016). This allows them to be released through the kidneys for elimination in urine, or into bile for elimination through the colon (Houston et al., 2016). The liver filters and detoxifies the blood coming from the digestive tract before it goes onto the urea which is then filtered out by the kidneys and flushed out the body (Houston et al., 2016). Manufacturing The livers lobules constantly produce bile and travels to the gall bladder shown in figure 1, for storage (Houston et al., 2016). Bile is important for breaking down fats threw the process of emulsification (Houston et al., 2016).The liver secrets three hormones Insulin-like growth factor 1 which stimulates cell growth, Erythropoietin which stimulates the creation of red blood cells by the red bone marrow and Angiotensin II which helps blood pressure control (Houston et al., 2016).When amino acids are absent from a person diet the liver produces proteins that are then secreted into the blood (Houston et al., 2016).The liver also produces plasma proteins (Houston et al., 2016). Storage The liver stores Vitamin A, B12, D, E and K until needed (Houston et al., 2016).The liver also stores essential minerals ion and copper (Houston et al., 2016).Energy is also stored as glycogen, which activates enzymes in the liver cells (Houston et al., 2016). When the bodies low on energy enzymes in the liver converts the glycogen to glucose a process called glycogenolysis, then releasing it into the blood stream (Houston et al., 2016). Sugars are passed into the capillary network and transported via the hepatic portal vein to the liver (Houston et al., 2016). The liver maintains a constant level of blood sugar by converting excess sugar to glycogen, so it can be stored (Houston et al., 2016). Amino acids are passed into the capillary network and transported via the hepatic portal vein to the liver (Houston et al., 2016). Metabolically active tissue need a large supply of amino acids for protein synthesis (Houston et al., 2016). Any excess amino acids received by the body can be converted in to glucose threw gluconeogenesis (Houston et al., 2016). Amino acids cant be stored, alternatively they are deaminated in the liver (Houston et al., 2016). Fats are absorbed as fatty acids and glycerol, these pass easily through the lipid biolayer of the plasma membrane active transport is not required for them (Houston et al., 2016). The majority of these molecules then recombine to make lipids which combine with proteins to make lipoproteins (Houston et al., 2016). Some excess cholesterol and phospholipids are eliminated in the bile (Houston et al., 2016). Q2 Having covered the gross anatomy, you are now ready to move towards lobule structure. Prepare a labelled diagram showing an overview of one liver lobule. This needs to identify all four fluid connections of the lobule. The hepatic artery, hepatic portal vein, hepatic central vein also known as the Inferior vena cava and the bile duct are the four fluid connections shown in figure 2. (Rolfe, 2018). The liver has two blood supplies one venous and one arteriolar (Rolfe, 2018). It has a blood supply suppling itself tis cell need oxygenated blood and glucose for cellular respiration (Rolfe, 2018). It also has a blood supply to work on and process that comes from the small intestine (Rolfe, 2018). The hepatic portal vein returns the blood to the heart (Rolfe, 2018). The Hepatic Portal vein carries blood from the spleen, pancreas and intestines and it contributes to 75 of blood volume to the liver (Rolfe, 2018). This blood is rich in nutrients but relatively poor in oxygen content (Rolfe, 2018). Hepatic artery is the branch of the celiac trunk from the abdominal aorta and it contributes to 25 of blood volume entering the liver, this blood is well oxygenated but nutrient poor (Rolfe, 2018). Blood from the portal vein and the Hepatic artery mixes as it enters and travels through a liver lobule (Rolfe, 2018) shown in figure 2. Blood then Penetrates the lobule and is collected by the Inferior vena cava and returned to the heart (Rolfe, 2018). Bile comprises the exocrine secretory product of the liver (Rolfe, 2018). It is stored in the gallbladder (Rolfe, 2018). During digestion bile is released from the gall bladder threw the cystic duct to the duodenum of the small intestine (Rolfe, 2018). Provide a labelled diagram of a segment from a liver lobule and indicate the flow of blood and bile. Blood flows within sinusoids from a portal vein to a central vein (Marieb, 2007) shown in figure 3. Bile flows within hepatic plates from the middle to bile ductules at the periphery of a lobule (Marieb, 2007) shown in figure 3. Secreted bile flows through tiny canals called the bile canaliculi these run between hepatocytes towards the bile duct branches in the portal triads (Marieb, 2007). The HYPERLINK https//www.78stepshealth.us/human-physiology/defecation.html o Defecation Human Physiology bile canaliculi are drained at the edge of each lobule by bile ducts, then the bile leaves the liver by the common hepatic duct (Marieb, 2007). Bile salts are reabsorbed into the blood by the ileum, returned to the liver via the hepatic portal blood then resecreted in newly formed bile (Marieb, 2007). This process is called enterohepatic circulation (Marieb, 2007). Blood and bile do not mix in the liver lobules as they travel in opposite directions (Marieb, 2007). Hepatocytes change cholesterol into bile acids (Marieb, 2007). These bile acids bind with more cholesterol, other lipids, electrolytes, water and the waste products from the destruction of old red blood cells known as bilirubin (Marieb, 2007).Every sinusoid passes through liver tissue comprising of two main cell types Kupffer cells and hepatocytes (Marieb, 2007).Kupffer cells are part of the sinusoid walls, they remove debris like bacteria and worn out red blood cells from the blood as it travels past (Marieb, 2007).Liver cells called hepatocytes is a chemical process that forms a thick epithelium that creates a functional barrier between two fluid compartments with distinct ionic compositions (Marieb, 2007). Part B (Assessment Criterion 2.2) Q3 How does the liver contribute to blood glucose homeostasis The regulation of glucose concentration in the blood shows how homeostasis is maintained by negative feedback (Toole and Toole, 2004). The cells of the islets of Langerhans reacts to a drop-in blood glucose levels by secreting a hormone called glucagon straight into the blood stream (Toole and Toole, 2004). Liver cells are the only cells that have the ability to bind to glucagon (Toole and Toole, 2004), causes liver cells to produce the enzyme phosphorylase (Toole and Toole, 2004).Phosphorylases purpose is to convert glycogen to glucose (Toole and Toole, 2004) by increasing the conversion of amino acids along with glycerol into glucose (Toole and Toole, 2004).Gluconeogenesis is to increase the amount of glucose in the blood and taking it back to its normal level (Toole and Toole, 2004).This increasing of blood glucose level makes the cells to minimalize the secretion of glucagon (Toole and Toole, 2004).Adrenaline is a hormone which can also increase blood glucose (Toole and Toole, 2004). When a human is stressed or excited the adrenal gland makes adrenaline (Toole and Toole, 2004). Adrenaline enables the breakdown of glycogen in the liver (Toole and Toole, 2004) this increasing the blood glucose level (Toole and Toole, 2004). If the glycogen supply in the liver becomes drained, cortisol is produced by the adrenal gland (Toole and Toole, 2004), enabling the liver to convert amino acids and glycerol into glucose (Toole and Toole, 2004). (Referenced from Body System Coordination WJF680 Graded assignment) Insulin and glucagon perform in opposite directions (Toole and Toole, 2004). Insulins job is to lower blood glucose (Toole and Toole, 2004), whereas glucagons is to increase it (Toole and Toole, 2004). This system is self-regulating (Toole and Toole, 2004) the amount of glucose in the blood determines the amount of glucagon and insulin produced (Toole and Toole, 2004). Fluctuation of glucose levels occur around a set point (Toole and Toole, 2004). The negative feedback process takes effect only when blood glucose levels drop below the set point, then the insulin secretion lowers (Toole and Toole, 2004), resulting in a rise in blood glucose (Toole and Toole, 2004). It the same for when levels go above the set point, (Toole and Toole, 2004), glucagon secretion lowers (Toole and Toole, 2004). (Referenced from Body System Coordination WJF680 Graded assignment) Q4 When there is continued high carbohydrate supply (especially fructose) but glycogen stores are already full, the liver maintains homeostasis by storing the excess energy in a different chemical form. Provide diagrams and brief annotations to analyse this process and its consequences. In homeostasis blood glucose control is a negative feedback loop (Brooker, 2008) shown in figure 4. The liver is a vital store of glycogen (Brooker, 2008). The liver can release Glycogen and break it down back into glucose this enabling the correction of blood sugar levels (Brooker, 2008) shown in figure 4. When glucose levels are too high the liver can absorb the glucose and convert it into glucagon for storage (Brooker, 2008). Once your glycogen stores are full, any excess carbohydrates or glucose are converted to glycogen and stored as fat or used in cell respiration for energy (ATP) shown in figure 5. A consequence of this would be obesity and possible diabetes (Brooker, 2008). Q5 The body cannot store amino acids. To maintain homeostasis, the liver therefore needs to eliminate excess amino acids. Use a diagram and brief annotations to analyse this process. Amino acids are proteins which are not stored in the body like carbohydrates and lipids therefore excess amino acids are destroyed by the liver cells in a process called deamination (Kang, 2012).Deamination removes amino groups from the amino acid to make ammonia (Kang, 2012) shown in figure 6.Threw enzyme catalysed reactions ammonia is mixed with carbon dioxide which makes urea (Kang, 2012). Urea then travels through the blood to the kidneys, then travelling out in the urine. Where as amination is the process of adding an amine group (Kang, 2012). Transamination is the swapping of only the amine group of the amino acid for a keto-acid group (Kang, 2012) shown in figure 7. Q6 Using chemical equations, analyse how the liver detoxifies drinking alcohol. Lobules in the liver filter and process chemicals, alcohol being one, but they only have limited regeneration properties (Whitney and Rolfes, 2013). Therefore, if the cells have to keep dividing and replenish constantly and rapidly eventually it gets worn out and too damaged to be able to repair itself resulting in fatty cells on the liver (Whitney and Rolfes, 2013). Alcohol upsets our water balance, it blocks the pituitary gland from releasing ADH (Whitney and Rolfes, 2013). Alcohol is metabolized by a two-step enzyme system called the alcohol dehydrogenase (ADH) pathway (Whitney and Rolfes, 2013) shown in figure 8. Firstly, the enzyme ADH converts alcohol to acetaldehyde generating NAPH H (Whitney and Rolfes, 2013). Acetaldehyde is toxic and requires immediate metabolization to something nontoxic (Whitney and Rolfes, 2013). However, if acetaldehyde builds up, some will pass from the liver into the blood (Whitney and Rolfes, 2013). Next in the ADH pathway is catalyzed by the enzyme acetaldehyde dehydrogenase (ALDH) (Whitney and Rolfes, 2013). This converts acetaldehyde to acetic acid (Whitney and Rolfes, 2013). The second pathway is the microsomal ethanol oxidizing system (MEOS), shown in figure 8, which is used when large volumes of alcohol are consumed (Whitney and Rolfes, 2013). It helps prevent blood alcohol concentration from getting dangerous high (Whitney and Rolfes, 2013). Part C (Assessment Criterion 2.3) Q7 The final aspect of liver function that you need to learn about is excretion. Do the following to prepare Define excretion. Excretion is where the body gets rid of waste specially the waste left from metabolism (Williams, James and Roberts, 2005). Everything we eat, and drink is for a reason one being we need energy to supply our cells (Williams, James and Roberts, 2005). Cells then undergo cell respiration leaving the waste product at the end of this cycle needing to be excreted immediately or broken down and changed into a nontoxic substance then excreted from the body, either as faeces or urine (Williams, James and Roberts, 2005). Provide a diagram that illustrates the urea cycle. Annotate this with briefly stating the reactants that go into the cycle, and the products that are coming out. The urea cycle also known as the ornithine cycle converts very toxic ammonia into urea for excretion see figure 9. References Brooker (2008). Biology. Dubuque, IA McGraw-Hill Companies, pp.1072-1073. Houston, Smith, Temple and Clifton (2016). How the body works. Dorling Kindersley, pp. 154,156-157. Kang (2012). Nutrition and metabolism in sports, exercise and health. Milton Park, Abingdon, Oxon Routledge, pp.173-174. Kent (2000). Advanced biology. Oxford Oxford University Press, p.139. Marieb (2007). Human anatomy physiology. 7th ed. 7th ed. Pearsons Benjamin Cummings, pp.912-915. McGuire and Beerman (2009). Nutritional sciences From Fundamentals to Food. Belmont, Calif. Wadsworth Cengage Learning, p.327. Rolfe (2018). RLO The Anatomy of the Liver Blood supply. online Nottingham.ac.uk. Available at https//www.nottingham.ac.uk/nmp/sonet/rlos/bioproc/liveranatomy/2.html. Waugh, Grant and Ross (2014). Ross and Wilson anatomy physiology in health and illness. Edinburgh Churchill Livingstone Elsevier, p.309,315. Whitney and Rolfes (2013). Understanding nutrition. Belmont Wadsworth, Cengage Learning, pp.222-223. Williams, James and Roberts (2005). Principles of Toxicology. Hoboken John Wiley Sons, pp.50-51. Assignment Brief Abingdon Witney College Access to HE -PNG – M6uD1 BDkkg( j/[email protected]_g D-(vmmm ( [email protected]/u
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